FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378335
Sequences flagged as poor quality0
Sequence length12-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT278060.6350816006541299No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA66710.15236385520980006No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA61390.1402131175435411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT98250.0102.607983
CTGCATA136000.0102.050381
CTGCTGC48250.0101.409921
CATAGTA95150.099.5301744
TGCATAG107750.098.8722462
CTGCTAT115350.096.85931
ATAGTAT95850.095.8729555
TGCTATA82900.092.992952
GCTATAA79200.091.823373
CAGCTGC38100.091.5495761
CAGCAGC41450.090.782341
GTATTCC102100.089.8226858
GCTGCTA7050.089.210243
AGTATTC105000.088.214117
CTGCAGC40800.088.0364151
TAGTATT108600.087.146236
GCTGCAC8700.084.222593
GCTGCAA8100.082.922873
TGCTGCA18050.080.884622
TGCTGCC17250.080.0321662
Produced by FastQC (version 0.11.5)