FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378335
Sequences flagged as poor quality0
Sequence length12-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCA294760.6732239538546045No Hit
GCGACTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGG71160.1625275361524415No Hit
GCGACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTC64890.148207023902922No Hit
GCGACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATTATTC47200.10780353718936536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGC2175800.0130.093292
GCGACTG2173200.0130.023511
CGACAGC2117400.0129.898652
GCGACAG2119350.0129.523011
GACTGCT732400.0123.646453
GACTGCA852950.0123.627793
GACAGCC679200.0122.221223
GACTGCG162650.0121.844893
GACAGCT642250.0121.162483
GACTGCC563700.0120.5486453
GACAGCA803150.0119.7684863
GACAGCG158950.0117.7818153
ACTGCTA187550.0112.9037864
CTGCTGC46900.0111.481915
ACTGCAT265450.0109.991944
ACTGCGT53450.0109.544214
GCATAGT98900.0108.922337
CTGCATA137150.0108.1327445
CATAGTA96000.0105.412428
ACTGCAG355900.0104.6155554
Produced by FastQC (version 0.11.5)