FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946487
Sequences flagged as poor quality0
Sequence length10-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT166580.5653512131565488No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC44000.1493303720668036No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA38130.12940834288425507No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT34840.11824250370016905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT61300.0100.154493
CTGCATA81000.097.3255161
CATAGTA59450.095.636324
TGCATAG65350.094.495852
ATAGTAT59150.092.991385
GCTATAA47450.089.489313
CTGCTAT68750.089.388251
CTGCTGC35550.089.037731
TGCTATA49600.088.500042
GTATTCC62700.088.104338
GCTGCTA9150.087.462423
AGTATTC63650.087.447617
AGCACTA42950.086.6288762
TAGTATT66000.084.9687966
CAGCTGC31300.083.191691
CAGCAGC40950.079.775811
CTGCAGC31300.077.467491
CTGCAGT98350.077.302191
CGATACG4850.075.51293130-131
CAGCACT102950.075.47251
Produced by FastQC (version 0.11.5)