FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16523970
Sequences flagged as poor quality0
Sequence length6-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT1265490.7658510636366442No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT362200.21919671846414632No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC337500.2042487368350342No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA264770.16023389052388742No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA196470.11889999800290124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT478050.095.593413
GCTGCTA55800.095.387323
CATAGTA460800.094.197454
CTGCATA622650.094.014761
TGCATAG501300.092.4727552
ATAGTAT461300.091.505415
CTGCTGC186050.090.038211
CAGCTGC132700.085.385781
GTATTCC496400.085.188328
TAGTATT514300.084.314356
CAGCAGC163400.084.3022841
AGTATTC513600.084.122597
CTGCTAT364450.083.447761
GCTATAA247450.082.0447163
CTGCAGC144400.080.874831
TGCTATA263450.080.506472
CTGCGTA51650.080.030681
AGCACTA235950.078.333942
TATTCCA567500.075.252329
GCGTAGT24600.074.84513
Produced by FastQC (version 0.11.5)