FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10745605
Sequences flagged as poor quality0
Sequence length9-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT385410.3586675668796685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC110700.089.481061
CTGCTGC109000.087.6274641
AGCTGCC32400.087.1034242
CAGCAGC118800.086.728121
GCATAGT155800.086.058673
CTGCAGC111650.083.498011
CTGCATA221850.083.159591
CATAGTA149650.082.933414
CTGCTAT220550.082.636871
GCTATAA141150.080.97953
TGCTATA147000.080.536522
ATAGTAT149800.080.086265
TGCATAG173850.079.599342
TGCTGCC36900.078.105172
AGCAGCC32600.077.210232
GCTGCTA14900.076.054963
TGCAGCC38600.074.947592
AGCTGCA41200.074.449482
AGCTGCT35950.073.6525652
GTATTCC167000.073.3062748
Produced by FastQC (version 0.11.5)