FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3816703
Sequences flagged as poor quality0
Sequence length14-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT194890.5106239599989835No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA43750.11462772974475614No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC38250.10021738657684394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT74300.0100.393583
CTGCATA100000.097.624791
CATAGTA72850.097.266814
TGCATAG80450.094.7730262
ATAGTAT74150.092.963425
CTGCTAT86350.090.6688461
TGCTATA58500.090.080182
GCTATAA56100.088.427593
GTATTCC77150.087.630628
AGTATTC79600.086.748597
TAGTATT81300.085.74926
CAGCTGC43700.084.8938751
CTGCTGC48900.082.516921
CAGCAGC54150.079.632741
CTGCAGC46400.079.4347461
CTGCAGT139250.077.805991
AGCACTA47650.076.59262
TATTCCA90100.075.1023569
CTATAAA59450.071.08914
AGCTGCC15750.070.740522
Produced by FastQC (version 0.11.5)