FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3764037
Sequences flagged as poor quality0
Sequence length14-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT191430.5085762972043049No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA45320.12040264216318808No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC38100.10122110914425125No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG37720.1002115547748335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT74050.0100.242323
CTGCATA99000.097.886171
CATAGTA71150.097.649074
TGCATAG80400.094.868972
ATAGTAT73100.092.5755165
CTGCTAT83000.090.5201641
GCTATAA56100.087.710483
GTATTCC79500.086.410358
CAGCTGC45400.085.592891
TGCTATA60350.084.424232
TAGTATT82750.083.669726
AGTATTC83000.083.636237
CTGCTGC48250.083.155221
AGCACTA46800.080.204682
CTGCAGC42850.079.596371
CTGCAGT132900.078.348941
CAGCAGC53850.077.635461
TATTCCA95900.072.700499
CAGCACT117950.070.5318451
CATACGT24100.066.702934112-113
Produced by FastQC (version 0.11.5)