FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12551078
Sequences flagged as poor quality0
Sequence length9-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT408930.32581265131170406No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA167180.13319971400066194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGC147700.088.615851
CAGCTGC129450.086.5146561
CAGCAGC146250.085.957851
GCATAGT180250.084.845143
GCTGCTA29400.082.221653
CTGCATA255000.082.201171
CTGCTAT254750.081.384221
CATAGTA174650.081.328724
CTGCAGC128000.080.178671
TGCATAG201250.078.994612
GCTATAA160300.078.592363
TGCTATA169700.078.08892
ATAGTAT178450.077.034145
CTGCAGT448550.073.422671
GCAGCAA42000.073.11063
TGCTGCG13550.072.726022
AGCAGCA74400.071.787132
AGCTGCC43850.071.516032
CTGCGGT79350.071.426811
AGCAGCC54200.069.4111562
Produced by FastQC (version 0.11.5)