FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366399
Sequences flagged as poor quality0
Sequence length12-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT50890.2150524911479425No Hit
ATACATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT32350.13670560205611987No Hit
ATCCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT25260.10674446701507227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT79850.0104.6591645
GGATTGC18250.0102.086754145
GTATTCC83050.0101.952878
AGTATTC84650.0100.681727
TAGTATT85100.0100.0791856
TATTCCA91700.092.134569
CATAGTA39600.092.091554
TCGACGC909.671777E-486.34232140-144
AATAGTA39900.080.147634
TATAAAG51050.075.647865
TAAAGAC56700.070.058987
AAGACAC57550.067.6337669
TATTCCG3850.065.482969
TATAGTA16500.063.734184
CCATAGT23750.063.7150343
TAAATAG35000.063.457512
ATAACCG2450.062.799411
TCGCCGA4300.062.374435125-129
ACATAGT24100.062.0239873
CGATAGT3450.060.6219983
Produced by FastQC (version 0.11.5)