FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897563
Sequences flagged as poor quality0
Sequence length6-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT342680.3462266418511304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN274490.2773308944838239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC29000.098.4735262
CAGCTGC100800.096.736671
AGCAGCC29250.093.987452
CAGCAGC112650.093.1348651
CTGCTGC87500.091.755791
CTGCAGC83450.088.275521
CTGCATA199100.088.20131
TGCAGCC26700.086.363512
GCATAGT143350.086.263443
TGCTGCC25550.085.639362
CATAGTA138200.083.30844
AGCTGCA33450.083.192712
AGCAGCA50150.082.115442
TGCATAG156750.081.857472
CTGCTAT176750.080.467491
ATAGTAT138850.080.053065
GCAGCAA27100.079.708443
AGCTGCT35550.078.751822
GCTATAA111150.078.644963
GCTGCTA13150.077.2263953
Produced by FastQC (version 0.11.5)