FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005322382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19829217
Sequences flagged as poor quality0
Sequence length6-126
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCGGGCGAGTCGCCTTCCCTCTCCCCTGGCCTCTCCTTCCCGCCTC1471760.7422179100667465No Hit
CAGCTTTTAGCCATGCCATGCTCTATAAATCACTTCCCTATCTCAGGTAG1263070.636974218397025No Hit
CTGCAGGGGGGACTGACACCGGGCCGCCGGGGTCCCTCCGGCCGGCCTGA1140150.5749848821564664No Hit
CAGCACCAGGCACTGCAGAGTGACCACTAACCTTCGCTCCATACCTAAGG1045380.5271917695993745No Hit
CAGCTTCTCTGTCATTCGATCCCAGGTACGATAGGGCAAGGGAGGAGCCC1032860.5208778541280777No Hit
CAGCCCGGGGTAGGTCCCCTGGACCTGCCGCTGGCGGACTACGCCTTCGT871510.43950802495126257No Hit
CAGCTCGTTGGAGAGGACCCGGCGAAGGGCGGGCCAGATTTGGAACTCGA856720.43204933407103263No Hit
CAGCGGCTTGACGCTCAAGGGCGGCCTGTAACTTAAGTGATGGTCTCCCT764960.3857741836200592No Hit
CAGCTGGCACTAAGGGCTCCTGCTGGTCAGCAGGGGCCGACAAAGCAAAT759240.38288955131208663No Hit
CAGCGGTGGCGGCGGCCCCGCGGACAGACGTCGAGGCATACTGCCGGTCC735580.37095766312910894No Hit
CAGCTTAAAGGTGCCGGCGGAAAAGGTCACGTGACACCTACGGCCACCTG709720.3579163009815264No Hit
CAGCTGGATGTACAGCTACACCGATCACCAGACGACTCCCACATTCGTAC704720.3553947692437881No Hit
CAGCACTTGAATCTGACTCCGATTGCGTAGCTCTAGCAGGGTAAAGAGCC689140.3475376763489955No Hit
CAGCCCACCCACACTTCCCGAGGATGCTACCCTTCTAATCAAATGGTTGG659640.3326606390963395No Hit
CAGCACCTGATCCGTGAAATCACCCCCATCGTGACGCATCGAGAGGCAAA643320.32443035950436167No Hit
CTGCTGTCTGGCTTACGTAAACGCGCTGGACTGAGAAGGTGGCCTAGCAA625300.31534275912155285No Hit
CAGCGGCTAGGTTCAGCCAGGCACCCGCGCCCCCAGCTAAGGCCAGGGCT624460.3149191417896128No Hit
CAGCGGGGAGTGACCATCACTATGCCGGTGGCCTCCGACTACCTCCCGGA605480.3053474073131582No Hit
CAGCCGCCAAGTGGATCCGGGGCATCTGCGAGGCCGTGCGGGGTCTTTCC602300.30374371312795656No Hit
CAGCTCCTGGCAGTCATTCATTCGGATAACGCCGAGTAGAAGCCGACGCA583450.29423753847668316No Hit
CAGCTTTGGCAGACCACCTGTGCTGGAAAGAGAGGCTGGAAGATGAGGCC568450.28667294326346826No Hit
CAGCAGTAATAGATGAAAACAAAGCTCAGCATGAAACAGCGTGGAGGCTC564850.28485744041229666No Hit
CAGCGTGGAGTTGTAGCGCCGCTGATTCTGGAGGATTAGCCGGAGTTCCC554040.2794058887953064No Hit
CAGCGCTACCTGCGTTTCCAAACGGCCTTTGATTACAACCAGGAGGACCT530250.2674084407871476No Hit
CAGCGAGGCCACCACGCTGGAAAGTGACTCGGGGCGGTGAAAGAGACCAC520210.26234520505776904No Hit
CAGCACCTCCCGTCCGGTCACGTCTCATGTTGGCATCATCGATCCCGGCT513460.2589411372118223No Hit
CAGCGGGGGGCTCATTGCTATGCTCGCCGATGCCGCCGAGAAGGACCTAT498790.2515429630932981No Hit
CTGCGCTCCTCCACCTCAAACACCAGGCCGGGGTGCTCTGCAAACATCTC476610.24035744830469102No Hit
CAGCCATGGCCCCCTCCTGGATGAAGAGCGAGGTATGCGGTGAGAGAATG463090.23353922648584663No Hit
CAGCACCGTCAAGGTGACGGTGCCCCAGGAGGCCTTTGTGGATCTGGACG460590.23227846061697746No Hit
CTGCGCACCAGTAGGTTGGCTAGCGTGCTGGCCCGCGCGGCATCTTCCTC451840.22786578007593541No Hit
CAGCGCCTCAGCCATTGCCAGCCTGGTTCCCTCCCTCAGATCCAACGACA448150.22600488965348456No Hit
CAGCCACTCGCGGGTCCCCGTAAAACATATGGAAAGGAATGGCGTGAAAG444840.2243356356431018No Hit
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT438310.22104251519361556No Hit
CAGCTTTACATAGAGGGTCTCCGTCATGTCATCAATGTTGGTCACCTTCA425250.2144562742946431No Hit
CTGCAGGGCAGGGTCAGCCCACGGCCGCGGACATCAAGGCCATGGCCGTG407510.2055098796891476No Hit
CAGCCCCTTCATCCTTTTGAAGGAGGCCTGCAACGCCCACTTTTGGCGCG397430.20042647170586714No Hit
CAGCTCCTTCTTGGGGTCCTTGCGCTGTGAGTTCTGCTCCAGATGGGCCA395340.19937247143949252No Hit
CTGCTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGA394340.19886816509194488No Hit
CAGCTGATCATCCTCTGTGTCAGTGGTGGAGGACAGAGGGAGGCGGCGGC382480.1928870918100296No Hit
CTGCTTGTCTCCGCAAAACCCGGCGGAGAGAACGTTAAGGTTTCAGGTAC381770.19252903430327079No Hit
CAGCGCCTCTACCAGAACATTTTCATGTGCTACCGCACGCTGGAGCATGC360160.1816309741327658No Hit
CAGCGCCTCAGACTCCCAAGAGGAAAAAGGGATTGGGCAAAGATAGCCCC354790.17892284904643488No Hit
CAGCCCTGCGCGCCAGACGGCCGCCGCACTCAGCGAGGAGGCCTGCGCGT353770.17840845657193624No Hit
CAGCACTCCTCCCTTGAGATGAGTCTGGGCATGGTCATAGCACTTGGACA353560.17830255223895125No Hit
CAGCCGTGACTAGCACGAACTGGGCCTCGTGGGAGCCAAAGGTGCTAATG351510.17726872422647852No Hit
CAGCTGGGGCCAGGGGAGTCGGCAGGTTACCCACCATTAGCCGGTGCACA348400.17570033148560532No Hit
CAGCTGGGGCTTCTTAGGCCAGAGCGCCGAGTCCGAGGCCAGCCTGGGCG345970.1744748670610645No Hit
CAGCTGAGACTCCATGGAGCGGATCTTCTTTAGATAGAGCTCCCTCTCCT344620.17379405349187516No Hit
CTGCAGGCCCAGCCCCCCGACGAAGCATACTCCACCGCCACCCCCGGCGC338590.17075308621616275No Hit
CAGCCAGGCGTTGAAGAACCAGTAGCAAAAGACCACGGCTGTTAGAATGT330900.16687497040352123No Hit
CAGCTTCGGCCTCGGCCATCTGGAGGCGGCCCTGGACGTGCTGGCATCCT326520.16466610860126246No Hit
CAGCGTGGTCAGGGTGGGCAGAGGACCATATTTCGCAGTCTGTATGCTCC321470.16211936154614678No Hit
CTGCGGAGTTGAGCCCGACGAACTCCTCCAGCAGTGGCGAGTTGAGTGTC315780.15924985842860057No Hit
CAGCTATGGACCACCAATGTGATCGTGGTGGACGAAGCTGGAACGCTTTC312070.15737888187919877No Hit
CTGCGTCCCAAGATCGTGGGCTGTCTGCGCCGCATCTACCGCGTTAACAT312030.15735870962529686No Hit
CAGCAGACATTCATCATTTAGAAATGTATCCAAGATTTCATTAAGTTCGG304290.15345537849527796No Hit
CAGCATCCCTGATATTCCTTGCGGTATATGGTGTGATGTCGTGTGGAGGC303720.1531679238771758No Hit
CAGCAGCTGGCCCCGAATCCCGCCGACAAGCGGACAGAGCAGACTGTTTT294430.148482917908458No Hit
CAGCACCTCAGCTGTTCCCAGTCTCCGACATAACCCAGAATCAACAGACT289800.14614797951931233No Hit
CAGCCATATGTGGACAGATGGACCTGAGCCACCCGCCCCCTCGTGGCCAT280100.1412562079481No Hit
CTGCCCTCTTCGATGATGCGCCGGTACGCGGCCGCCAGGGCCCGGGTCTC276630.13950626492210963No Hit
CAGCTGGTCAAGAGCGGCATCGTGCTGTCAGGGCATGACATCAGCGACGG274860.13861364268695026No Hit
CTGCACCCCCTTTATGATATTTACATTGGCAGGGATGCCGGGGAGAGGGC268370.13534069449136593No Hit
CAGCGCCATCATCTCCCGTTGTTCCAATAGAATAACTTCCCGGTCTGTGG262200.13222912432699688No Hit
CTGCACAGGGCCTAAAGAAAAAGGTCTCCAACACGGTGGTCAAGTGTCTG261630.1319416697088947No Hit
CAGCGGGCCAATTCGTAGAGCCACCAAGTGGCATCAGCCTCAAGGATGGC260530.13138693272659227No Hit
CAGCTTGGCCAGACCCTCGGTGGCCACCTGGCGCAGGTACTGCTCCTTGC255590.12889565936970682No Hit
CAGCATGCAGGCTCGGGCGCGGGCCGTACTTTGGTTGTGGACCAGGCCCA253710.12794756343631722No Hit
CAGCGGTTGGGCCGAGGCGAGGAGGAGGCGTCCCGGGAGAGCCCGGAGGT252400.1272869221210298No Hit
CAGCTCCTGTATTTAACTTTGCGGACAGAGGCCAGAGCCGTTTGCTCCAT250610.12638421375891948No Hit
CAGCCGGATGCACCGCCCGGCGTGATACACGCCGCTGTCAAAAAGCGGGG248750.12544620395248085No Hit
CAGCCCGCACACCGCTCACCAGGTGGCAATTCTACCTCTAAACAGGAGGA246090.12410474906800406No Hit
CAGCGCTCAAATTGGTGATGAGACAAGTAGCGGATGATAAGGCGAAACAT233830.11792195324706971No Hit
CAGCAGTGACTCGGGCAACAGTGACACCGAGCAGAGCGAGCGGGAAGAGG224410.11317138745317074No Hit
CAGCTGTCTCCCCGGGCTGGCCAAATAGACGGTTGCGGTCCACCAGGTTG223930.11292932040634787No Hit
CAGCCGATAGCACCCTAGATCTAGTGAATCATGGCGAGCCCGGAAGAGAG223910.11291923427939692No Hit
CTGCCCTGACGGCCATCGTGAGCGAGTACGTGGATCGGACGCTACCCGAA222240.11207704267899232No Hit
CTGCCTTGTCCGGCCATCTTTTAGACACGGAAGACAATGTGCCGCCATGG219150.11051873606507004No Hit
CAGCGACTTTCCGCGCCTGCCTCATGACACTCGCACAGCCCACACCCTTT217550.1097118459089938No Hit
CAGCAACTATGCATCGCAATAGGGAGCCAGTTGGGGAGGAGGGGAACTGG213890.10786608467696934No Hit
CTGCTTGTGATCATGGAAACCACTCAGACTCTCCGCTTTAAGACCAAGGC213680.10776018034398434No Hit
CAGCCACGCCACGCGGCCTCTGGCATCCCCCTGTGGCTGTTTCTTAGCGC210450.10613127084140538No Hit
CAGCAACGACGGGGAAGACGCGCGCCGCCTCACCCGCCACCGCATCGCCG209340.10557149079562747No Hit
CAGCTCTAACTTGGCATCTCTTTTACAGTGGTTCTCCTGATTTGCTCTTC208960.10537985438355936No Hit
CAGCGCTAACATGATGGCTTGTAATTCTGTCTTACTAATGGGATTAATGC206090.10393249516609758No Hit
CTGCTTCGGGAATACGTCTCTGCGCCCAAAGCCCTGATGGAAGACCTGGC204230.10299448535965894No Hit
CAGCGGTGCTCTATACCGCTTCGTCACCTGTTGCAACTGCACCCTGCCCA203180.10246496369473389No Hit
CAGCCTGGAGGTCTGGCAGGTGCCTGACGTCTGAGAAAAAGTCCTGGGTG202980.10236410242522435No Hit
CTGCCCGGCCAGGGCCATCTCCACCAGGCAGGTCACCAGGCCCCCGTCGC199940.10083101112867945No Hit
CTGCAGCACCTCGGCGCACAGGTTGGCGGCGTTCATGGCCTCGCCCTGCA199860.10079066662087566No Hit
CAGCTGAGGGGCAACCTGAACTTTACCAACTCCGTCTCCCTAAAGCTGAT199480.10059903020880755No Hit
CAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGGCTA199260.10048808281234706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)