FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377526
Sequences flagged as poor quality0
Sequence length6-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT189040.795112230108104No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA57300.24100682810619106No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATTATTCACAA41960.17648597744041494No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC29790.12529831429814017No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGTGGCACTATTCACAA28270.11890511397141398No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT24830.10443629217934947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT66650.0104.205143
CATAGTA63400.0102.642724
TGCTATA65000.0102.148562
GCTGCTA7800.0101.4110263
CTGCATA84550.0101.280181
GCTATAA64300.0100.837523
CTGCTAT82200.0100.749311
ATAGTAT63850.099.94855
CTGCTGC30100.098.7463461
TGCATAG72500.098.2765052
CAGCTGC23950.096.079681
GTATTCC66500.094.8882758
AGTATTC67950.094.186287
TAGTATT69150.093.503096
CTATAAA65200.090.52664
CAGCAGC27500.089.778091
CTGCAGC23550.088.80561
AGCACTA39250.088.551142
TGCTGCC10850.085.606712
TATAAAG64500.085.2845845
Produced by FastQC (version 0.11.5)