FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377526
Sequences flagged as poor quality0
Sequence length6-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACTGATATCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTC244671.0290949499605893No Hit
CCACACTGATATCTGCTATAAAGACACATGCACACGTATGTTTATTGCGG118850.4998893808101363No Hit
CCACACTGATATCTGCTATAAAGACACATGCACACGTATGTTTATTGTGG48070.2021849603327156No Hit
CCACACTGATATCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCC35990.15137584194662856No Hit
CCACACTGATATCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGAT31240.13139709092560922No Hit
CCACACTGATATCTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTT25340.10658137913107996No Hit
CCACACTGATATCTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGT23820.10018817880435378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA53550.0361.50644145
TATAGCA44000.0340.05402145
TGATATC2354600.0125.5156257
ACTGATA2349100.0125.500075
CTGATAT2356900.0125.443136
CACTGAT2355100.0125.027754
GATATCT1193100.0124.9890068
GATATCA1173600.0124.977198
ATATCAG1174800.0124.806519
ACACTGA2351050.0124.720233
CCACACT2352400.0124.487921
ATATCTG1199250.0124.348029
CACACTG2357950.0124.2939452
ATATCCG4250.088.9619759
ACCGGTA1955.3558324E-876.85824145
CTACGCA3102.8194336E-1064.46175145
TACTATG50400.063.58546140-144
ACTATGC51250.061.77016140-144
ATACTAT51850.061.280937140-144
CGGATAT3250.060.1354566
Produced by FastQC (version 0.11.5)