FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697747
Sequences flagged as poor quality0
Sequence length16-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGGCGAGCATACTGCATAGTATTCCATGGTGTATATGTGCCACATTT320120.6814330358786882No Hit
ACCAGGCGAGCATACTGCTATAAAGACACATGCACACGTATGTTTATTGC116700.24841695391429128No Hit
ACCAGGCGAGCATACAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCA78900.16795285058986786No Hit
ACCAGGCGAGCATACTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAA70860.15083826353356194No Hit
ACCAGGCGAGCATACAGCACTATTCACAATAGCAAAGACTTGGAACCAAC50070.10658300670512907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATG52650.0434.56702145
TTGCGTC6000.0200.99518145
CGAGCAT4627750.0121.337197
GCGAGCA4633100.0121.2724466
CAGGCGA4627700.0121.147323
AGCATAC4650800.0121.092479
GGCGAGC4643350.0121.083315
AGGCGAG4644050.0121.033644
GAGCATA4656500.0120.96128
CCAGGCG4656650.0120.649982
ACCAGGC4602800.0120.290391
CGCATAC15150.099.058469
GCGCATA15850.094.683638
CGCGCAT16700.089.86447
CGAGTAT7550.073.2321247
AGCGTAC6300.071.367159
TACTACG3303.2960088E-971.05891145
ATACTAT54450.069.39598140-144
TTTATAG33900.066.207855145
AAGGCGA22650.065.208243
Produced by FastQC (version 0.11.5)