FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4695312
Sequences flagged as poor quality0
Sequence length23-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT231510.49306627546795617No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC72720.15487788670912603No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG64490.13734976504223786No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA54820.11675475452962444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT92250.097.400683
CATAGTA89150.095.544294
CTGCATA122950.095.467931
ATAGTAT88000.093.5785755
TGCATAG101950.090.7373352
CTGCTAT107650.089.791631
GCTATAA71000.089.001133
TGCTATA75200.088.174282
AGTATTC96500.086.829077
GTATTCC96050.086.461288
TAGTATT98250.085.459616
CTGCTGC61500.082.949211
CAGCTGC54750.081.159941
AGCACTA59350.080.607142
CTGCAGC57650.079.279521
CAGCAGC69900.078.842671
TATTCCA112900.074.732879
CTGCAGT155750.073.065771
TACGTGT28950.072.587685114-115
CGTATAA11000.070.29148114-115
Produced by FastQC (version 0.11.5)