FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053223
Sequences flagged as poor quality0
Sequence length10-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGCCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCC194520.4799143792483167No Hit
CATGCCTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATT47050.11608046238758638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCCA2004700.0126.1179051
ATGCCAG2008750.0126.1038062
CATGCCT2087500.0125.5510941
ATGCCTG2095800.0125.147972
TGCCAGC2035150.0124.6554263
TGCCTGC2124750.0123.55543
GCCTGCA812250.0117.906564
GCCTGCT708350.0116.44464
GCCAGCA752750.0116.112364
GCCAGCT607750.0115.603074
GCCAGCC688800.0113.27314
GCCTGCG179400.0111.4298864
GCCAGCG171950.0109.001054
GCCTGCC604900.0107.543794
CCTGCTA163050.0105.037855
GCATAGT76900.0104.693368
CCTGCAT236850.0102.1718145
CTGCATA110150.0101.8942956
CATAGTA73200.0101.071149
CTGCTAT95600.098.939816
Produced by FastQC (version 0.11.5)