FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15219140
Sequences flagged as poor quality0
Sequence length6-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT512230.33656960905806765No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA201560.13243849521063608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC165400.095.827821
CAGCAGC185550.094.948711
AGCAGCG15500.093.505122
TGCAGCG12000.090.583092
AGCTGCG12350.090.238592
GCAGCGA9750.089.527053
CTGCTGC138850.089.3994751
CTGCAGC145350.088.158891
AGCTGCC47650.087.561632
GCTGCTA19750.087.559893
TGCTGCG9600.086.9231642
GCATAGT247800.086.114253
CTGCATA320800.085.705811
GCAGCAA44050.085.494853
AGCAGCC57450.085.143372
AGCAGCA86650.084.328172
GCAGCAC24350.084.0952763
CATAGTA234850.083.850464
GCAGCGG11050.082.969023
AGCTGCA67550.081.759042
Produced by FastQC (version 0.11.5)