FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038958
Sequences flagged as poor quality0
Sequence length16-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT36840.12122576225140327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT62250.095.489285
AGTATTC66900.088.6565257
GTATTCC66200.088.555488
TAGTATT67650.087.950796
CGGACTA5000.078.78072
TATTCCA79550.074.321499
ACGATCA7050.071.89062145
CATAGTA27800.069.924454
AATAGTA36200.067.857444
TATAAAG51400.062.1400075
TAAAGAC55450.057.595687
CGTACTA2100.056.569672
GATAGTA16500.054.928474
CGAACTA2550.053.9425962
ATAAAGT40200.053.8437651
ATAACCG4300.053.829291
AAGACAC58550.052.944189
ACTATTC55400.050.319765
CCGTACT3500.050.0466881
AACTATT25900.049.7142564
Produced by FastQC (version 0.11.5)