FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615855
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT81800.094.270925
GTATTCC83650.090.820268
TAGTATT88150.089.1236046
AGTATTC87800.088.970977
CGGACTA8200.073.151592
TATTCCA103450.072.887819
TATAAAG83550.069.0334555
CATAGTA38000.067.922514
TAAAGAC88550.066.056177
CGTACTA3250.066.0555952
CGCACTA7450.063.5651442
CTTTGAA64050.061.276985145
GATAGTA24400.060.778784
AAGACAC95950.060.3691679
AATAGTA43800.058.3518834
ATAACCG6500.052.4781071
TATAGTA26300.052.0688484
GCCCTCG27050.050.910156145
AAGCTCG17000.050.8497859
CCGGACT15700.050.6954081
Produced by FastQC (version 0.11.5)