FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066449
Sequences flagged as poor quality0
Sequence length12-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTCGACTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAA188410.6144240455327971No Hit
CTGGTCGACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACT41010.1337377533427101No Hit
CTGGTCGACTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGA33190.108235943268582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC3025050.0127.800183
GTCGACA1546600.0127.725964
TGGTCGA3016300.0127.710782
GTCGACT1475200.0127.669874
CTGGTCG3009750.0127.6613161
TCGACAG1548200.0127.651795
TCGACTG1475900.0127.596325
CGACAGC1552200.0127.339346
CGACTGC1481950.0127.278076
GACTGCT495800.0121.230357
GACTGCA565550.0121.019077
GACAGCC495750.0120.5591057
GACTGCG113450.0119.9689257
GACAGCT461100.0119.6085747
GACTGCC395000.0118.7945567
GACAGCA580000.0118.555147
GACAGCG124800.0115.256527
ACTGCTA120400.0109.0526358
TCGACCG7350.0107.8881155
ACTGCAT173050.0107.050568
Produced by FastQC (version 0.11.5)