FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001346
Sequences flagged as poor quality0
Sequence length11-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT676770.4511395177472742No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA154280.10284410478899693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC161100.089.5964661
GCATAGT300850.088.951123
CAGCAGC191550.088.589531
CATAGTA285900.086.94244
GCTGCTA32850.086.448953
CTGCATA372150.085.918531
CTGCTGC136250.083.615991
TGCATAG309250.083.496022
ATAGTAT293700.082.003085
AGCACTA255200.081.481262
GCTATAA209550.081.053133
CTGCTAT299000.079.844441
TGCTATA219200.078.159162
AGCTGCC49750.077.754272
CTGCAGC125800.076.592651
GTATTCC319500.076.556148
AGCAGCC67450.076.386352
CAGCACT544400.074.993551
AGTATTC334850.074.3302157
TAGTATT332950.074.329556
Produced by FastQC (version 0.11.5)