FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396223
Sequences flagged as poor quality0
Sequence length13-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT682660.4433944610960753No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA156390.10157686076643603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC197850.090.2708361
CAGCTGC170250.089.475951
GCATAGT290650.088.449263
GCTGCTA36150.087.5575563
CATAGTA277750.086.05654
CTGCATA371400.085.472831
CTGCTGC142750.083.892191
TGCATAG303850.082.8885352
ATAGTAT283950.081.859975
AGCACTA247850.081.0124362
AGCAGCG18950.080.357752
GCTATAA202550.079.691243
CTGCTAT295150.079.1408541
AGCTGCG15800.078.479582
CTGCAGC129400.078.047321
GCAGCAA49850.078.0045243
TGCTATA212300.077.901412
GTATTCC314500.075.9107068
AGCAGCA102000.075.605782
AGCTGCC52100.075.262132
Produced by FastQC (version 0.11.5)