FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918539
Sequences flagged as poor quality0
Sequence length10-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT152310.5218707031154971No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA44960.1540496803366342No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA37180.12739250700436075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT65900.0100.7634053
CATAGTA63100.099.1856164
CTGCATA87050.097.860881
CTGCTAT75500.095.818631
GCTATAA51100.094.377813
TGCTATA53000.094.312812
CTGCTGC38450.093.9166261
TGCATAG72000.093.660242
ATAGTAT65600.092.8212365
GTATTCC70150.086.976488
CAGCTGC29150.086.8820951
AGTATTC73500.084.825817
CGATACG2900.084.50648130-131
CTGCAGC33600.083.489971
TAGTATT75450.082.150276
CTGCAGT117600.081.506411
CTGCGTA8050.080.534231
CAGCAGC33200.079.2034761
GCTGCTA7350.079.133723
CTGCGTG28150.076.4086461
Produced by FastQC (version 0.11.5)