FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10203389
Sequences flagged as poor quality0
Sequence length12-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT452180.4431664812544146No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG141690.13886562592095628No Hit
CTGCTGTCTACTTTCTATTTGTAATCCCGTTTCCAACGAAATCCTCAGAA139510.1367290808965531No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC126740.12421363137287032No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA113000.11074751731998064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT185600.090.410653
CTGCATA245600.088.488551
CTGCTGC116500.088.227531
CATAGTA179750.087.354614
TGCATAG199350.085.533622
ATAGTAT179700.085.117565
CAGCTGC99900.084.881221
CTGCTAT225450.084.837371
CTGCAGC109950.083.873951
TGCTATA151850.082.602392
CAGCAGC113300.082.493921
AGCTGCC28700.082.12282
GCTATAA145100.081.814693
TGCTGCC36550.080.643232
GTATTCC198350.077.0596858
TGCAGCC37950.076.240522
AGTATTC205650.075.167387
TAGTATT208750.074.700016
AGCAGCC30150.073.5008542
AGCAGCA53350.072.818392
Produced by FastQC (version 0.11.5)