FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782965
Sequences flagged as poor quality0
Sequence length15-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT440890.4506711411111049No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA134590.1375758780696854No Hit
CTGCTGTCTACTTTCTATTTGTAATCCCGTTTCCAACGAAATCCTCAGAA119580.1222328813401663No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG112690.11519002674547031No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA111450.11392251735542344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT174450.091.716063
CTGCATA243350.088.914581
CATAGTA171400.087.968394
TGCATAG195150.086.0919342
CTGCTAT219200.085.2855451
GCTATAA144400.084.6120453
ATAGTAT173950.084.554055
TGCTATA151550.084.1195762
CTGCTGC144700.077.761511
GTATTCC190400.077.277048
CAGCTGC121250.075.998261
CTGCAGT382300.075.863381
AGTATTC195300.075.847817
TAGTATT196150.075.659866
CTGCAGC148450.074.6442261
CAGCAGC136100.071.6411
AGCACTA106350.071.382912
CTATAAA150850.066.683564
TATTCCA223450.066.316619
CTGCTGT400750.065.881751
Produced by FastQC (version 0.11.5)