FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782965
Sequences flagged as poor quality0
Sequence length15-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT463270.4735476412314672No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC138200.141265965890709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT165950.093.6198963
CATAGTA160500.089.957734
CTGCATA221300.088.955171
TGCATAG184050.086.750172
ATAGTAT162950.086.4517065
CAGCTGC132050.081.782991
GCTATAA125200.081.461713
AGCACTA138850.081.035172
CTGCTAT182150.080.6674961
GTATTCC175700.080.5647358
TAGTATT178200.079.783856
AGTATTC180750.079.034037
TGCTATA129050.078.8998642
CAGCAGC155700.076.449331
CTGCTGC117450.072.865481
CAGCACT341750.072.376231
CTGCAGT335400.071.873311
CAGCTTT259850.071.053311
ACACCAT194350.070.01477120
CTGCAGC117150.069.283511
Produced by FastQC (version 0.11.5)