FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8967051
Sequences flagged as poor quality0
Sequence length7-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT411930.4593817967579307No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA109460.12206911726051295No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA91920.10250861738156725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT179900.088.496353
CTGCATA229950.088.051711
CATAGTA172550.086.764624
TGCATAG194300.084.139262
CTGCTAT191000.082.9226761
CAGCTGC99500.082.841021
ATAGTAT176200.082.166175
GCTATAA132150.081.1314243
TGCTATA135150.081.075372
AGCACTA119250.077.0310752
CAGCAGC117300.076.581771
CTGCAGC90350.076.359311
GTATTCC191650.075.628128
CTGCTGC96000.074.550191
AGTATTC199300.073.853357
TAGTATT201750.072.684686
CTGCAGT316500.072.255651
AGCAGCG10650.071.0622562
CTGCGGT55300.070.708871
CAGCACT304100.068.385091
Produced by FastQC (version 0.11.5)