FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16551020
Sequences flagged as poor quality0
Sequence length6-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT824480.4981445252316775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC182250.092.613271
GCATAGT329650.092.104863
CAGCAGC207350.090.796961
CTGCATA415400.089.302191
CATAGTA318150.089.222854
TGCATAG348850.087.359162
AGCACTA281300.086.741772
ATAGTAT324200.084.846225
CTGCTGC135200.083.117591
CTGCTAT328400.081.621421
GCTATAA234950.080.29793
CAGCACT635850.079.794991
GTATTCC349150.079.1015248
TGCTATA245950.077.874742
CTGCAGC135300.077.771531
AGCAGCG21350.077.26432
AGTATTC365150.077.0546657
TAGTATT365650.076.4428256
AGCTGCC56950.075.6272
GCTGCTA23600.075.3841863
Produced by FastQC (version 0.11.5)