FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15719176
Sequences flagged as poor quality0
Sequence length22-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT615480.39154724140756486No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG212420.1351343098391417No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC192070.12218833862538342No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA160310.10198371721265798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC39950.099.882192
AGCAGCC42800.097.0365142
GCTGCTA23900.096.995193
CAGCTGC148250.096.170311
TGCTGCC40700.095.50722
TGCAGCC43200.095.3839952
CTGCTGC155000.094.294071
CAGCAGC167050.094.284921
CTGCAGC133600.092.289371
GCAGCAA34900.090.6908343
GCTGCTT23450.088.669893
GCTGCAT15600.087.35133
GCATAGT247100.087.22443
CTGCATA328750.086.042481
GCAGCAT20650.084.918593
CATAGTA236400.084.833954
GCTGCAA22950.084.451193
AGCTGCA51200.084.403532
AGCAGCA76150.084.197172
CTGCTAT329300.082.832141
Produced by FastQC (version 0.11.5)