FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10446636
Sequences flagged as poor quality0
Sequence length6-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT497870.476584041025264No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477350.4569413541354366No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA106460.10190840381535261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC119600.093.359151
CAGCAGC129250.092.783161
GCATAGT208350.091.68783
CTGCATA255650.089.7709961
CATAGTA198600.088.885734
TGCATAG219000.086.9781652
AGCACTA176150.086.464392
ATAGTAT200350.085.4147345
AGCTGCG10400.082.171492
CTGCTGC83350.081.348691
CTGCTAT198350.080.783291
AGCAGCG11950.079.981942
GCTATAA138900.079.693923
GTATTCC215300.079.4649668
CAGCACT393600.079.362621
AGCTGCC35100.078.647572
CTGCAGC85450.078.0993651
TAGTATT221700.078.058296
AGTATTC223850.077.838477
TGCTATA145400.077.2577442
Produced by FastQC (version 0.11.5)