FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528159
Sequences flagged as poor quality0
Sequence length10-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT270440.5972405120933253No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC63290.13976982698708237No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG61990.1368989030641371No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA51090.1128273101717497No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA50550.11163477254221858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT102600.0102.074613
CTGCATA136200.099.891651
CATAGTA97200.099.828424
ATAGTAT96450.097.9254765
TGCATAG111600.096.1053542
GTATTCC103550.091.56158
CTGCTAT102450.091.192851
TAGTATT106900.090.602366
GCTATAA69100.090.223083
AGTATTC106850.090.083117
TGCTATA73050.088.3895652
CTGCTGC55500.084.168531
CAGCTGC51550.082.220891
CTGCAGC53800.081.3524861
CAGCAGC64850.078.893521
TATTCCA121100.078.3445059
CTGCAGT157850.077.4690251
AGCACTA48700.073.822572
CTATAAA74400.069.897254
CTGCGTA11700.068.341971
Produced by FastQC (version 0.11.5)