FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15900251
Sequences flagged as poor quality0
Sequence length20-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT520400.32729043082401654No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC244200.1535824811822153No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG239300.15050076882434119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC159950.094.221211
AGCTGCC44400.093.606262
CAGCAGC175950.092.0447851
CTGCAGC149100.089.964241
CTGCTGC152000.089.24861
TGCTGCC42450.087.283692
AGCAGCC50100.085.5590062
CTGCATA294750.084.639781
TGCAGCC48500.084.348952
GCATAGT212650.083.747153
GCTGCTA19200.083.4732363
CTGCTAT307700.081.730871
AGCAGCA74550.081.547172
CATAGTA202600.081.412064
AGCTGCA56800.081.1099242
GCAGCAA40900.079.167953
GCTATAA192800.078.5619353
GCTGCTT27250.078.352443
TGCATAG238350.078.341312
TGCTATA203450.077.627182
Produced by FastQC (version 0.11.5)