FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15900251
Sequences flagged as poor quality0
Sequence length20-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT546880.34394425597432393No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC302050.18996555463181053No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG196270.12343830295509171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC48350.096.751392
CAGCTGC172750.095.374251
CAGCAGC194750.095.307721
AGCAGCC50750.092.945012
CTGCTGC133050.088.924661
CTGCAGC127600.087.522961
GCATAGT218400.086.212483
TGCTGCC40150.084.849122
AGCAGCA86250.084.555212
CTGCATA298950.084.188871
TGCAGCC43600.082.866732
AGCTGCA60550.082.782262
CATAGTA208350.082.7437064
GCAGCAA41050.082.038083
GCTGCTA17950.082.003283
TGCATAG233400.080.6957242
AGCTGCT57350.080.498652
ATAGTAT208200.079.962635
CTGCTAT264100.078.3033141
GCTATAA170950.076.6135563
Produced by FastQC (version 0.11.5)