FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14316875
Sequences flagged as poor quality0
Sequence length6-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT511220.35707512987296464No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA214700.14996289343868688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC150600.085.525771
GCATAGT210250.085.493013
CTGCATA300300.083.599341
CTGCTGC157350.081.995751
CAGCAGC172000.081.363361
CATAGTA200700.081.342464
CTGCTAT281650.080.9119641
TGCATAG233750.078.510262
TGCTATA188700.078.4976352
GCTATAA182050.078.034813
ATAGTAT202250.077.748485
CTGCAGC142100.077.072331
GCTGCTA29600.074.3621753
CTGCGGT89200.071.708431
CTGCAGT488600.071.292051
TAGTATT228850.069.904596
AGTATTC233650.069.567187
GTATTCC232700.069.147038
AGCTGCC54200.069.0287552
CTGCGTA32650.068.9274441
Produced by FastQC (version 0.11.5)