FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14316875
Sequences flagged as poor quality0
Sequence length6-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT510610.35664905923953377No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA190620.13314358056489284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT15100.0100.48644120
CAGCTGC157150.088.058131
GCATAGT208300.086.853253
CAGCAGC179000.084.8736951
CTGCATA289500.084.384061
CATAGTA197050.083.713614
ATAGTAT196650.081.05485
CTGCTGC140800.080.8056641
TGCATAG228400.080.5382162
CTGCTAT259750.079.015731
GCTATAA171800.076.918993
TGCTATA177500.076.538052
CTGCAGC134400.076.20911
AGCAGCG17150.073.384332
GCTGCTA29800.073.1534653
CTGCAGT477550.072.815031
GTATTCC224850.072.5237358
AGCTGCC54400.071.367192
TAGTATT229000.071.276426
AGTATTC230900.071.0182957
Produced by FastQC (version 0.11.5)