FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15967070
Sequences flagged as poor quality0
Sequence length9-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT523370.32778086399070083No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC242080.15161203652266822No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG239670.15010268007843644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC162350.092.901441
AGCTGCC46400.092.153262
CAGCAGC177650.091.200611
AGCAGCC46350.090.259912
CTGCTGC151200.089.870511
CTGCAGC145700.088.154241
TGCTGCC43850.087.847742
GCTGCTA19900.085.1834953
GCATAGT217450.085.1518553
TGCAGCC47850.084.137622
CTGCATA308250.083.740821
AGCAGCA80750.081.884552
CATAGTA209300.081.224344
CTGCTAT304500.080.8109741
AGCTGCA60050.080.615512
GCTGCAA29450.078.776473
GCAGCAA41200.078.4645843
GCTATAA193700.078.229913
TGCATAG243650.077.957692
TGCTATA204000.077.822292
Produced by FastQC (version 0.11.5)