FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15967070
Sequences flagged as poor quality0
Sequence length9-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT546920.3425299694934637No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC306120.19171958286648708No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG195890.12268374848986069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC47000.096.905352
CAGCTGC170050.096.578321
CAGCAGC197400.095.369061
AGCAGCC56150.094.219342
CTGCTGC137300.089.7315751
GCTGCTA17250.089.195073
CTGCAGC130800.086.007641
GCATAGT218550.085.9862063
CTGCATA297150.085.961291
AGCAGCA84950.085.5732042
TGCTGCC40200.085.1463242
GCAGCAA39150.084.581533
CATAGTA207450.083.546484
AGCTGCA62850.082.401292
AGCTGCT55500.081.86312
TGCAGCC43300.080.595352
ATAGTAT209450.080.033895
TGCATAG239400.079.871812
CTGCTAT273550.078.887831
GCTGCTT26050.078.7518843
Produced by FastQC (version 0.11.5)