FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6540035
Sequences flagged as poor quality0
Sequence length6-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT281840.4309457059480569No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC163070.24934117325060184No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT144230.2205339879679543No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA77560.11859263750117546No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA73720.11272110929069952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT116950.086.412373
CTGCATA155150.084.768611
CATAGTA111500.084.573924
GCTGCTA29250.084.434953
TGCATAG124800.082.707442
GCTATAA88100.082.339583
ATAGTAT111650.081.882615
CTGCTAT132200.080.299691
TGCTATA94650.079.976272
CTGCTGC85450.079.2569961
CAGCTGC85550.078.711251
AGCACTA95500.077.93082
CAGCAGC97100.075.6218951
TAGTATT124800.074.676316
GTATTCC125650.073.9507758
AGTATTC129200.072.776057
CAGCACT232850.071.274421
CTGCAGT205900.070.060721
CAGCGGT31000.069.488211
CTGCGGT33800.069.3021851
Produced by FastQC (version 0.11.5)