FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300323
Sequences flagged as poor quality0
Sequence length13-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT624100.5522850983993998No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT299060.2646473025594047No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC212520.1880654207848749No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA162150.14349147365079742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA49350.097.594093
GCATAGT258350.093.002393
CTGCATA341150.091.521
CTGCTGC160000.090.644441
CATAGTA249150.090.3915944
TGCATAG277500.089.044272
ATAGTAT253800.086.814345
CTGCTAT284750.086.4354251
CAGCTGC99700.085.2590561
GCTATAA190650.084.7951353
TGCTATA201250.084.420722
CAGCAGC112150.083.1371
CTGCAGC122550.081.165631
GTATTCC275800.079.987568
AGTATTC281450.079.402037
TAGTATT285250.078.610166
TGCAGCG12500.077.580392
TGCTGCG8600.076.2247242
CTGCAGT409300.075.6859361
TGCTGCT103850.074.860772
Produced by FastQC (version 0.11.5)