FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298261
Sequences flagged as poor quality0
Sequence length23-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT60500.093.2030645
TAGTATT62200.091.260676
AGTATTC63700.090.703147
GTATTCC62000.090.542148
CGTACTA2400.076.3363952
TATTCCA74150.076.3011259
CATAGTA29850.069.173144
CGCACTA4300.069.051692
ATCCACA39600.067.45981145
CGGACTA4250.063.9181862
CGCGATA1750.061.3932461
TCGACAC3800.060.603638145
AATAGTA34500.060.215864
TCGACGC3200.059.53765140-144
GATAGTA18150.057.0560044
CGCGTAT1400.054.1705061
GTCTACT21650.053.9545636
CCGGTTT8400.053.4181371
TATAAAG50900.051.4830675
CGGGATA6400.050.3616451
Produced by FastQC (version 0.11.5)