FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14708886
Sequences flagged as poor quality0
Sequence length6-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT503200.34210612550807723No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA189610.12890847070267591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC164700.094.330331
CAGCAGC180150.093.531321
CTGCTGC129150.090.0410161
AGCAGCG14100.088.473382
CTGCAGC144450.088.303261
AGCTGCC45050.088.19612
GCATAGT249050.086.894163
AGCTGCG15250.086.846642
CTGCATA319350.086.3368761
GCTGCTA20850.085.92373
CATAGTA238300.083.826874
AGCAGCC57950.083.641352
TGCTGCG9400.083.6014942
TGCAGCG14150.083.500272
GCAGCAA44500.083.35763
ATAGTAT235050.082.227115
AGCAGCA87700.082.212932
TGCATAG269600.081.657662
GCTGCAA28250.081.312873
GCTGCGG13000.080.317783
Produced by FastQC (version 0.11.5)