FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4251385
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTACTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCC235300.5534666937950808No Hit
AGCTACAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAA67220.15811317958735802No Hit
AGCTACTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCT65100.15312656934152047No Hit
AGCTACTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATT53010.12468877789238095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTGC2097950.0126.455133
CTACAGC2157950.0126.4043053
AGCTACA2146400.0126.2328951
GCTACTG2103850.0126.085252
GCTACAG2163250.0126.02942
AGCTACT2103450.0125.416661
TACTGCA792550.0123.834094
TACAGCG136600.0122.5795754
TACTGCG132500.0122.50064
TACAGCC676650.0122.488364
TACAGCA780700.0122.33764
TACTGCC527450.0122.1084
TACTGCT715500.0121.7799764
TACAGCT645300.0121.601724
TCGACGC3950.0112.61323145
ACTGCAT246200.0110.078735
GCATAGT88950.0108.1083158
ACTGCTA165650.0107.973955
CTGCATA120350.0104.603546
CATAGTA87200.0103.297299
Produced by FastQC (version 0.11.5)