FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285892
Sequences flagged as poor quality0
Sequence length11-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT274190.5187203976169018No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG82110.15533802052709364No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC80800.15285972547301382No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA66730.1262417014952254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT103700.0101.32613
CTGCATA143250.097.877121
CTGCTGC60150.097.744741
CATAGTA101850.097.483934
ATAGTAT101850.094.612425
TGCATAG117000.094.041582
CAGCTGC54750.093.212631
CTGCTAT125450.092.685351
GCTGCTA7600.091.857163
CTGCAGC51300.091.6857151
GCTATAA82700.090.980933
TGCTATA87450.090.133842
GTATTCC110600.086.64098
CAGCAGC60150.086.432151
AGTATTC113200.085.8105247
TAGTATT116450.083.877576
TGCTGCC20350.083.271962
GCTGCAA10150.078.1855243
TGCAGCC20050.077.9707642
AGCACTA59800.077.828432
Produced by FastQC (version 0.11.5)