FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4257036
Sequences flagged as poor quality0
Sequence length20-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT225880.5306039225414114No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC66960.15729253875231497No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG62660.14719161407138676No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA49540.11637204853329876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT87250.099.906143
CATAGTA83800.098.28934
CTGCATA119400.097.893231
CAGCTGC44200.095.346741
CTGCTAT100000.095.212551
ATAGTAT85150.093.981345
TGCATAG96250.093.8076252
GCTATAA64850.092.294583
CTGCTGC47400.091.9494861
TGCTATA69300.091.305812
GTATTCC90400.090.384928
CAGCAGC51950.090.136371
AGTATTC92250.089.677617
TAGTATT92000.089.464526
CTGCAGC43250.087.724721
AGCTGCC13350.079.1448062
AGCACTA52500.078.786342
AGCAGCC17000.078.043072
GCTGCTA4850.077.9811863
CTGCAGT155200.077.9038241
Produced by FastQC (version 0.11.5)