FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249996
Sequences flagged as poor quality0
Sequence length13-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT267510.5095432453662823No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG81940.15607630939147382No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC79900.15219059214521305No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA67100.12780962118828282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT104050.0100.6244353
CATAGTA99450.098.0160754
CTGCATA142650.096.117171
CTGCTGC61550.094.7497941
CTGCTAT124900.094.2923361
TGCATAG114300.093.793322
CAGCTGC50600.093.194081
ATAGTAT101200.092.901825
GCTATAA85550.092.3114853
TGCTATA89350.091.324172
CTGCAGC50700.087.0058061
GTATTCC108800.086.5802468
CAGCAGC57500.086.578481
AGTATTC112750.084.7639857
TAGTATT113600.084.495186
TGCTGCC19500.082.343532
GCTGCAA10550.080.909493
TGCTGCA22500.078.527362
GCTGCTA7100.078.188073
CTGCAGT176650.076.231991
Produced by FastQC (version 0.11.5)