FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249996
Sequences flagged as poor quality0
Sequence length13-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT97950.097.228095
GTATTCC102700.091.690268
TAGTATT105550.091.462866
AGTATTC105250.091.366947
CATAGTA54350.079.351154
TATTCCA121600.077.436859
TATAAAG86050.061.5016335
CGTACTA3900.059.233742
CGCACTA7100.058.9180642
CGCGATA1600.058.5517651
TAAAGAC91550.057.3252837
TATAGTA32500.057.0358434
AAGACAC94850.054.4076779
ACGATCA11300.053.66232145
CGCTATA5950.052.4833681
AATAGTA47950.051.0237354
GATAGTA25600.050.710534
CCATAGT36300.050.042593
GTCTACT31350.049.9630436
TCGTATA4250.049.9484522
Produced by FastQC (version 0.11.5)