FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199845
Sequences flagged as poor quality0
Sequence length6-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCC281610.541573835373939No Hit
TCAGCCTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCT84250.1620240603325676No Hit
TCAGCCAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAA77690.14940829967047095No Hit
TCAGCCTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATT69110.13290780782888723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC4300.0130.61586145
TCAGCCA2610100.0123.53211
AGCCTGC2657500.0123.203753
AGCCAGC2652850.0122.412163
TCAGCCT2668900.0121.922021
CAGCCAG2744000.0118.39942
GCCTGCA988900.0116.238454
CAGCCTG2825600.0115.9012762
GCCTGCT925750.0115.650944
GCCAGCT823650.0114.97014
GCCAGCA958100.0114.216094
GCCAGCC912950.0111.172624
GCATAGT101200.0107.237538
GCCTGCC807650.0106.2298364
CCTGCTA218950.0105.523915
CATAGTA97650.0104.414479
CTGCATA138750.0103.994696
GCCTGCG206750.0103.821424
CTGCTGC57500.0103.505976
GCCAGCG208350.0102.77924