FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113836
Sequences flagged as poor quality0
Sequence length6-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT66220.3132693359371304No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA21880.10350850302483258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG1750.090.62899130-131
GCATAGT30150.089.244243
CATAGTA28200.086.646364
CTGCTAT42050.086.111361
GCTATAA27900.085.889793
CTGCATA41650.085.100651
CAGCTGC26900.082.73531
TGCTATA29650.082.607592
TGCATAG33000.082.607292
AGCACTA30450.081.9841462
CTGCTGC25450.079.814651
ATAGTAT29800.079.2281345
CAGCAGC31050.076.03861
CAGCACT72450.074.3590851
GTATTCC32550.073.8007058
AGTATTC33600.073.421987
TAGTATT33350.072.560016
CCGATAC2200.071.665764128-129
CTGCGTA3800.069.723671
CTGCAGT62550.068.5261
Produced by FastQC (version 0.11.5)