FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113836
Sequences flagged as poor quality0
Sequence length6-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC1253.1795207E-7101.13938140-144
ATAGTAT42900.094.542385
TAGTATT46100.087.979796
GTATTCC44550.087.348568
AGTATTC45950.086.811047
CATAGTA21750.074.5924454
TATTCCA53400.074.128669
GATAGTA12200.059.492144
CGCGATA1051.8189894E-1258.1343461
TAGAACG1752.0449639E-457.793934140-144
AATAGTA22550.057.0700654
CAATCGA1852.691075E-454.669937140-144
GATCGGA7400.054.10931145
CCGTAGG4000.053.4109271
GGATTGC15100.053.03429145
CGGACTA3300.051.7691152
CCGTACT2600.051.650131
CGCACTA2600.051.6268962
TATAGTA10800.051.391364
TAGCGTC2003.9543502E-450.56969140-144
Produced by FastQC (version 0.11.5)