FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841643
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT82550.098.09375
AGTATTC88200.093.814167
GTATTCC89600.091.736138
TAGTATT90700.091.023976
CATAGTA42200.082.052334
TATTCCA104850.079.5011149
CGTACTA2450.077.301082
CGCACTA6000.064.147432
ACGATCA7200.064.01245145
TATAGTA24050.063.2272954
CCGTACT6550.060.6445961
GATAGTA19850.058.4538884
AATAGTA38850.056.7461624
GTCTACT25950.056.710846
TATAAAG60850.056.5018235
CCGTATG4750.055.3216251
TAAAGAC62650.054.9737937
CGCGATA1800.054.3209721
CGGTATA3750.052.1481361
CGGACTA4450.050.796252
Produced by FastQC (version 0.11.5)